Figure 7
From: Identification of nuclear-enriched miRNAs during mouse granulopoiesis
The effect of miR-706 inhibition on the expression of putative target miRNAs and mRNAs in MEL and MPRO cells. (A) Representative plots showing high efficiencies of transfection of Dy547-labelled inhibitor control in MEL and MPRO cells (right) compared to non-transfected controls (left), indicating that miR-706 hairpin is likely to be transfected at similar high efficiencies. (B) Immunofluorescent microscopy of MEL cells showing cytoplasmic localization of transfected inhibitor control (red), with nuclei counterstained by Hoechst 33342 (blue). (C) Expression of miR-706, miR-142-3p, miR-532, miR-192 and miR-194 in cells transfected with miR-706 hairpin inhibitor compared to control. (D) Expression of Stat1 in MEL and MPRO cells transfected with miR-706 inhibitor and control. (*P < 0.05; **P < 0.01, t-test).