Figure 1

Effect of miRSNPs on miRNA binding and protein expression. A-C) Jurkat cells were transfected in triplicate with 1 µg either empty psiCheck2 vector or psiCheck2 constructs containing 3′UTRs with wild-type and variant alleles, with miRNA mimics, inhibitors or miRNA negative control (50 pmol/well). 24 h post transfection luciferase activity was measured. Data show relative Renilla luciferase levels normalized to firefly luciferase and corrected for the effect of miRNA mimics on the empty psiCheck2 vector. Values for the miRNA negative control were set as 100%. All transfections were done three times. * p < 0.05, ** p < 0.01, *** p < 0.001. Sequence alignments of D) miR-18a-3p with the ARHGAP26 3′UTR, E) miR-34c-5p and miR-449b-5p with the ETV6 3′UTR and F) miR-510-5p and miR-589-3p with the PML 3′UTR. MiRNA seed sequences are underlined. Allelic variants in each 3′UTR are underlined and in bold. For miR-510-5p its seed sequence repeated twice is shown (one in bold, the other underlined) instead of the entire mature miRNA sequence to show its complementarity with two adjacent miR-510 binding sites in the PML 3′UTR.