Effects of Shb deletion on disease progression in murine model of CML. (a) Kaplan-Meier curve demonstrating survival of mice receiving either wild type or Shb knockout BCR-ABL transformed bone marrow from 5-FU treated mice. (b) Analysis of various disease parameters including liver and spleen weight as well as weight loss at the end-stage of the disease. (c) Bone marrow cell numbers from the tibia, femur and the iliac bones were determined at the time of death. (d and e) Bone marrow cells were stained with fluorescently labeled antibodies directed against Gr-1 and Mac-1 and subsequently analyzed with FACS for GFP (BCR-ABL), Mac-1 and GR-1. Plots are representative of a typical experiment. Data are presented as mean ± SEM and based on 15 mice of each genotype from 5 independent experiments (retroviral transformation and transplantation occurring at 5 separate occasions). *denotes p < 0.05 as determined by Student’s t-test.