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Figure 3 | Journal of Hematology & Oncology

Figure 3

From: The Src homology-2 protein Shb modulates focal adhesion kinase signaling in a BCR-ABL myeloproliferative disorder causing accelerated progression of disease

Figure 3

Proliferation and apoptosis in BCR-ABL+hematopoietic progenitors assessed by flow cytometric analysis of Ki-67 and cleaved Caspase-3. (a, b and c) The cell cycle status in BCR-ABL+Lin-c-Kit+ and BCR-ABL+Lin+ bone marrow was examined by staining for proliferation marker Ki-67 in combination with the DNA binding dye Hoechst 33342. FACS plots are representative of an average experiment. (a and d) The staining for presence of cleaved Caspase-3 was used to determine the percentage of apoptotic cells within the BCR-ABL+Lin-c-Kit+ population. FACS plots are representative of a typical experiment. Data are presented as mean values ± SEM with 9 mice from each genotype from 3 independent experiments. *denotes p < 0.05 as determined by Student’s t-test.

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