Figure 7

Selinexor (KPT-330) reduces migration and invasion of DU145, PCb2 and PC3 tumor cells. (A) migration test with treatment with 50 nM KPT-330 by using Boyden chambers and osteoblast conditioned medium as chemotractant. (B) invasion test in the same experimental conditions. (C) Deregulation of MMP-9, MMP-2 and uPA secretion after selinexor treatment. (D) time-dependent variation in the levels nuclear and cytoplasm levels of P27 and ILK after treatment with selinexor. Each Western Blot lane was loaded with 40 μg of proteins and normalized vs b-actin (cytoplasm levels) and lamin B (nuclear levels). (E) VEGF measured by ELISA and performed on conditioned media from cells treated or not with 20 or 50 nM selinexor. (F) Il-8 measured by ELISA and performed on conditioned media from cells treated or not with 20 or 50 nM selinexor.