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Figure 6 | Journal of Hematology & Oncology

Figure 6

From: Developing aptamer probes for acute myelogenous leukemia detection and surface protein biomarker discovery

Figure 6

Competition binding assays of aptamer K19 and anti-Siglec-5 antibody. For blocking aptamer binding with antibodies, NB4 cells were pre-incubated with anti-Siglec-5 or isotype control antibodies before binding to biotin-labelled aptamers K19 (a) or E10 (b). The fluorescence intensities of bound aptamers were determined by flow cytometry. Similar approaches were employed with aptamers K19 (c) or E10 (d) used to block binding of the FITC-anti-Siglec-5 antibody on NB4 cells. The FITC-isotype control (IgG1) was used as a negative control for specific antibody binding.

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