Figure 7

Using Siglec-5 as a biomarker for granulocytic different maturation in human bone marrow. Leukocytes from normal bone marrow aspirates were incubated with aptamer K19 or the single-stranded negative control DNA. (a). Siglec-5 expression of maturing granulocytes. The granulocyte population was first identified using levels of side-scattered light (SSC) in combination with the fluorescence intensity of CD45. Then, maturing granulocytes were separated into three subsets according to the expression levels of CD13 and CD11b (left panel): early stage (yellow), immediate stage (purple), and matured stage (green). Fluorescence intensity (PE) of the granulocyte subsets bound with single stranded DNA control (middle panel) or aptamer K19/Siglec-5 (right panel) is shown in relation to fluorescence levels of CD11b (APC). (b). Siglec-5 expression of maturing monocytes. The mature and immature monocytes were first identified using levels of side-scattered light (SSC) in combination with the fluorescence intensity of CD64. Then, maturing monocytes were separated into three subsets according to the expression levels of CD64 and CD14 (left panel): early stage (yellow), immediate stage (teal) and matured stage (fuchsia). Fluorescence intensity (PE) of the monocyte subsets bound with single-stranded negative control DNA (middle panel) or aptamer K19/Siglec-5 (right panel) is shown in relation to fluorescence levels of CD14 (APC).