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Figure 6 | Journal of Hematology & Oncology

Figure 6

From: Interferon-γ-induced upregulation of immunoproteasome subunit assembly overcomes bortezomib resistance in human hematological cell lines

Figure 6

Effect of IFN-γ after siRNA knockdown of immunosubunits PSMB8 (β5i) and PSMB9 (β1i). (A) PSMB8 mRNA expression after PSMB8 siRNA with and without 100 U/ml IFN-γ for 24h compared to non-target siRNA with and without 100 U/ml IFN-y, (B) PSMB9 mRNA expression after PSMB9 siRNA with and without 100 U/ml IFN-γ for 24 h compared to non-target siRNA with and without 100 U/ml IFN-γ. Results are presented as percentage relative to controls (mean (± SD) of 3 individual experiments). (C) β5i protein expression after PSMB8 silencing for 48 h compared to non-target control siRNA (58% downregulation) and protein expression of the mature protein form of β1i after PSMB9 silencing for 48 h compared to non-target control siRNA (78% downregulation). (D) Chymotrypsin-like activity of non-target siRNA as compared to PSMB8 or PSMB9 siRNA with and without 100 U/ml IFN-y for 24 h. Results are the means of 4 separate experiments. *P < 0.05

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