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Table 2 Primers for JAK2 Exon 12, JAK2 V617F and CALR mutation screening and sequencing

From: The mutation profile of JAK2 and CALR in Chinese Han patients with Philadelphia chromosome-negative myeloproliferative neoplasms

 

Primer

Sequence (5’-3’)

Amplicon length (bp)

JAK2 exon 12 mutation screening

E12HRM-F

AATGGTGTTTCTGATGTACC

127

 

E12HRM-R

AGACAGTAATGAGTATCTAATGAC

 

JAK2 exon 12 sequencing

E12SEQ-F

CTCCTCTTTGGAGCAATTCA

496

 

E12SEQ-R

GAGAACTTGGGAGTTGCGATA

 

JAK2 V617F

V617F-F

AGCTTTCTCACAAGCATTTGG

150

 

V617F-R

TGACACCTAGCTGTGATCCTG

 
 

V617F-P

AAATTATGGAGTATGTTTCTGTGGAGACGAGA

 

CALR exon sequencing

CALRExon1-F

CGGGTGGGTATAAAAGTG

348

 

CALRExon1-R

GGGACGCAGAAGAGAAAT

 
 

CALRExon2-F

GTTGGAATGGGGAGTGTC

337

 

CALRExon2-R

CTTCCTCCACCTGTCCTC

 
 

CALRExon3-F

CGGTGACGAGGAGAAAGATA

493

 

CALRExon3-R

TAAGAAAGTCAATGGGGTCT

 
 

CALRExon4.5-F

GTTTCTCTTCTCAGCCTTG

678

 

CALRExon4.5-R

TCTCTTCATCCCAGTCCTC

 
 

CALRExon6.7-R

ATCCCACAGACTCCAAGC

611

 

CALRExon6.7-R

GGACTTCACAGGGACAGAC

 
 

CALRExon8.9-F

CGGTGTTCCTTGTCTTCTC

584

 

CALRExon8.9-R

ACAGAGACATTATTTGGCG

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