Figure 2
Schematic representation of delivery systems used for iPS induction: viral, non-viral; integrative, integration but excisable, integration-free approaches and the challenges encountered. Non-viral delivery integration-free methods have been successfully employed to produce integration-free iPSCs. Insertion of small pieces of vectors and transgenes into the genome of “integration-free” hiPSC lines and potential immunogenicity against transplanted iPSCs as well as other challengers prevent iPSCs from clinical applications.