The MCC-interactome in human MM cells identified by affinity purification followed by LC-MS/MS. The human MM cell line 8226 cells were transduced with lentiviruses expressing hMCC-SBP-6xHis or FLAG-hMCC. Immunoprecipitates of hMCC-SBP-6xHis by streptavidin-sepharose beads from whole cell lysates and purified mitochondria of 8226 cells were analyzed by high resolution LC-MS/MS, respectively. Immunoprecipitates of FLAG-hMCC by streptavidin-sepharose beads were used as negative control in these experiments. (A) Venn diagram of MCC-interactors identified in the present study and previous studies. The diagram depicts the number of previously known MCC-interactors or MCC-interactors identified in whole cell lysates or mitochondria of human MM cells in our study, as well as the overlap between each category. (B and C) Functional clustering and annotation of MCC-interactors identified in whole cell lysates (B) or mitochondria (C) of human MM cells. The number of proteins of each functional cluster is shown. (D) Representative mass spectral profile of a peptide of PHB2 identified by LC-MS/MS. The deduced peptide sequence is shown. (E) Schematic diagram of peptide sequences of PHB2 identified by LC-MS/MS. The peptide sequences detected by LC-MS/MS are highlighted in green color.