Figure 8

PARP1 and PHB2 are two hubs of the MCC interaction network in human MM cells. The human MM cell line 8226 cells were transduced with pUB-hMCC-SBP-6xHis or pUB-FLAG-hMCC. Immunoprecipitation was performed as described in Figure 7. (A and B) Immunoblot analyses of proteins pulled down by streptavidin-sepharose beads (SA IP) from whole cell lysates (A) or purified mitochondria (B). Proteins were immunoblotted for PARP1, PHB2, SBP, and followed by MCC. Blots of an aliquot of whole cell or mitochondrial lysates before immunoprecipitation were used as the input control. (C) The MCC interaction network in human MM cells. We performed an interaction network analysis using the top 100 MCC-interactors and the STRING analysis tool (http://www.string-db.org). The top 100 MCC-interactors identified in our study and used for the interaction network analysis include 60 proteins identified in both whole cell lysates and mitochondria, 10 proteins identified in mitochondria only, and 30 proteins identified in whole cell lysates only. Sixty-one proteins of the top 100 MCC-interactors of human MM cells form an interaction network centered at PARP1 and PHB2.