Figure 6From: Small-molecule survivin inhibitor YM155 enhances radiosensitization in esophageal squamous cell carcinoma by the abrogation of G2 checkpoint and suppression of homologous recombination repairYM155 enhances radiation induced apoptosis. Cells were seeded into six-well plates at the concentration of 2 × 105/mL, incubated with 10 nmol/L YM155 or 0.1% DMSO for 24 h, and (or not) irradiated at dose of 10 Gy, respectively. Forty-eight hours after incubation, cells were harvested. A, The cells were stained with AnnexinV/FITC and propidium iodide and examined by flow cytometry for apoptotic cell populations. Data were Mean ± SD (n = 3). **, P < 0.01. B. Western blot analysis of apoptosis-related proteins. Actin was used as a loading control.Back to article page