Figure 3

Effects of FENDRR on gastric cancer cell migration and invasion in vitro and in vivo. MGC803 cells were transfected with pcDNA-FENDRR, and BGC823 cells were transfected with si-FENDRR. (A) qPCR analysis of FENDRR expression levels following the treatment of MGC803 cells with empty vector and pcDNA-FENDRR (left panel), and the treatment of BGC823 cells with scrambled siRNA and si-FENDRR (right panel). Experiments were performed in triplicate. Bars: SD; **p < 0.01. (B) Forty-eight hours after transfection, MTT assays were conducted to determine the proliferation of MGC803 and BGC823 cells. Experiments were performed in triplicate. Bars: SD. (C) Colony-formation growth assays were conducted to determine the proliferation of MGC803 and BGC823 cells. The colonies were counted and photographed. Experiments were performed in triplicate. Bars: SD. (D) Wound healing assays were used to investigate the migratory ability of gastric cancer cells. Experiments were performed in triplicate. Bars: SD; **p < 0.01. (E) and (F) Transwell assays were used to investigate the changes in the migratory and invasive abilities of gastric cancer cells. Experiments were performed in triplicate. Bars: SD; *p < 0.05 and **p < 0.01. (G) MGC803 cells transfected with pcDNA-FENDRR and empty vector were separately injected into the tail veins of athymic mice. Lungs were harvested from the mice in each experimental group, and the numbers of tumor nodules visible on lung surfaces were counted. The assay was independently conducted twice. Bars: SD; *p < 0.05 and **p < 0.01. (H) Visualization of the entire lung, and hematoxylin and eosin (HE)-stained lung sections.