Figure 4

FENDRR regulates FN1 expression and MMP2/MMP9 activity. (A) Expression of cell adhesion molecules (FN1, integrin, CD44, ICAM-1, E-cadherin, N-cadherin and vimentin) as detected using qRT-PCR after FENDRR was overexpressed in MGC803 cells or (B) blocked in BGC823 cells. (C) After FENDRR is overexpressed in MGC803 cells, western blot analysis shows that the FN1 protein level is diminished, as compared to the level in the control group. (D) The FN1 protein level is elevated after FENDRR expression is blocked in BGC823 cells, as compared to the level in the control group. (E) and (F) FENDRR regulates the enzymatic activity of MMP2/MMP9 in gastric cancer cells. All the above experiments were performed in triplicate. Bars: SD; *p < 0.05 and **p < 0.01.