Translation of sequencing into Clinical Oncology & Research. Patients start with informed consent to access their archived tumor sample. When applicable, circulating tumor cells and plasma DNA are sampled; as well as accessible fresh tumor. Tumor DNA is then purified and sequenced, and the results are presented at a tumor board of experts, where the patient is then allocated to an appropriate trial based on the results. Fresh tumor can also be grafted into mice to determine if a theraphy is effective, before giving to the patient. Tumor resampling after progression on theraphy is essential to identifying the mechanism of resistance and a new treatment strategy.