Figure 1
From: Notch1-induced T cell leukemia can be potentiated by microenvironmental cues in the spleen
Distribution of leukemia cells in different organs in the Notch1-induced mouse T-ALL model. After injection of T-ALL cells, the size and weight of different organs and the population of T-ALL cells in these organs were measured at the indicated time points. (a) The weight of different organs was measured and calibrated by the respective body weight. (b) The typical sizes of the thymus and spleen are shown. (c) The population of T-ALL cells was determined by flow cytometry analysis and shown as cell counts per 104 cells. (d) The phenotype of T-ALL cells was determined by flow cytometry analysis. (e) Two-photon fluorescence microscopy was used to track the distribution of leukemia cells in mice femurs (left) and spleen (right) within three days. GFP+ T-ALL cells are indicated by arrows. All the data are plotted as the means ± SD, and the statistical analysis was performed using Student’s t-test (**, p < 0.01; ***, p < 0.001).