Figure 5

HDACIs downregulated NF-κB signaling through HDAC1. A, Expression of Class I HDACs including HDAC1, HDAC2, HDAC3 and HDAC8 were detected by western blot in Jurkat and H9 cells treated with VPA (0.5 mM) and SAHA (2 μM) for 72 h. B, HDAC1 and c-FLIP expression were determined by tissue array. *, P < 0.05 comparing with B-cell lymphomas. C, HDAC1 expression was evaluated by immunohistochemical study on tumor sections of PTCLs patients according to c-FLIP expression. The histogram represented the percentage of HDAC1-positive tumors. *, P < 0.05 comparing with low c-FLIP group. D, HDAC1 enzymatic activity was assessed by ELISA in Jurkat and H9 cells treated with VPA (0.5 mM) and SAHA (2 μM) for 72 h. *, P < 0.05 comparing with the untreated (Control) group. E, Cell growth inhibition of Jurkat cells transfected with Con siRNA or HDAC1 siRNA treated with different concentrations of HDACIs (VPA and SAHA) was determined by MTT assay. *, P < 0.05 comparing with the negative control (Con siRNA). F, HDAC1, P50 and c-FLIP expression were detected by western blot in Jurkat cells transfected with Con siRNA or HDAC1 siRNA.