Figure 1
From: Intratumoral anti-HuD immunotoxin therapy for small cell lung cancer and neuroblastoma
Western blot analysis of HuD proteins in SCLC, neuroblastoma, and leukemia/lymphoma cell lines. Protein extracts from cell lysates were subjected to SDS-polyacrylamide gel electrophoresis (SDS-PAGE) on a NuPAGE™ 4-12% Bis-Tris gel, transferred to nitrocellulose membrane. The membrane was probed with anti-HuD mAb (16A11) and a HRP-conjugated goat anti-mouse IgG detection system. The neuroblastoma (Neuro-2a) and SCLC (NCI-H69, DSM79) extracts demonstrated strong bands at 40 kD, whereas two control cell line (BW5147, K562) extracts were devoid of signal.