Figure 1

hBM-MSCs protect ALCs against cytotoxic drugs by up-regulation of gal-3 at both the mRNA and protein levels. (A) ALCs were treated with IDA (25 nM for Reh and Jurkat cells, 20 nM for Sup-B15, 15 nM for Kasumi-1) or VP-16 (250 nM for Reh cells, 200 nM for Sup-B15, 1000 nM for Jurkat), cultured with or without hBM-MSCs. The relative number of surviving ALCs was analyzed with CCK-8 assay, as indicated. Absorbance of ALCs cultured alone were defined as 1. (B) The apoptotic level of ALCs treated with IDA or VP-16, cultured with or without hBM-MSCs was determined by FACS. (C) ALCs cultured with hBM-MSCs expressed higher level of gal-3 and β-catenin, compared with those unconditioned.