Figure 2
Comparison of reconstitution capacity of HSC in Angptl7 −/− Angptl7 +/− and Angptl7 +/+ mice. (a) Experimental design for assessing the repopulation efficiency in Angptl7-null BM environment. (b) Comparison of the repopulation efficiency in BM of Angptl7 −/− Angptl7 +/− and Angptl7 +/+ recipients. Data represent the means +/− s.e.m. (n = 8 for each group). (c, d) Comparison of the frequencies (c) and number (d) of LT-HSCs in BM of Angptl7 −/− Angptl7 +/− and Angptl7 +/+ recipients. Data represent the means +/− s.e.m. (n = 8 for each group). *P ≤ 0.05 for bar 9 versus bar 7 and bar 8. (e) Comparison of the second transplantation repopulation efficiency in BM of Angptl7 −/− Angptl7 +/− and Angptl7 +/+ recipients as referred in (a). Data represent the means +/− s.e.m (n = 7 for each group). *P ≤ 0.05 for bar 6 versus bar 4 and bar 5, for bar 9 versus bar 7 and bar 8, for bar 12 versus bar 10 and bar 11. (f) Experimental design effect of endogenous deletion of Angptl7 in HSCs. Angptl7 −/− Angptl7 +/− or Angptl7 +/+ donor BM CD45.2 cells (5x105 cells) were transplanted into lethally irradiated WT CD45.1 recipient mice. (g) Comparison of the repopulation efficiency of donor BM Angptl7 −/− Angptl7 +/− and Angptl7 +/+cells. Data represent the means +/− s.e.m. (n = 8 for each group). (h, i) Comparison of the frequencies (h) and number (i) of donor Angptl7 −/− Angptl7 +/− and Angptl7 +/+ LT-HSCs in BM of WT recipients. Data represent the means +/− s.e.m. (n = 8 for each group). (j) Comparison of the repopulation efficiency of Angptl7 −/− Angptl7 +/− and Angptl7 +/+ donor BM cells in second recipients mice as described in (f). Data represent the means +/− s.e.m (n = 8 for each group).