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Figure 2 | Journal of Hematology & Oncology

Figure 2

From: Insulin like growth factor binding protein 7 (IGFBP7) expression is linked to poor prognosis but may protect from bone disease in multiple myeloma

Figure 2

IGFBP7 is silenced via methylation. (A) MM cell lines were treated with DMSO (0.1%) or 5-aza-2′deoxycytidine (500 nM) for 96 hours. Trichostatin A (50 ng/ml) was added for the last 24 hours of incubation. Subsequently IGFBP7 expression was analysed by quantitative PCR. Values represent the mean ± SD of three independent experiments performed in triplicates. Asterisks indicate statistical significance compared with the control (* P < 0.05, ** P < 0.01, *** P < 0.001). (B) Pyrosequencing was performed along 328 base pairs within the IGFBP7 promoter region containing 37 CpG sites (schematic representation). The graph on the left displays the percentage of methylation of each individual CpG site for all samples analysed. The graph on the right represents the corresponding IGFBP7 expression values in the samples used for pyrosequencing. Cycles indicate the mean percentage of DNA methylation along all 37 CpG sites in the promoter region analysed.

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