Figure 2

Lack of IGF-IR alters leukemia development. (A) Expression of mRNA encoding BCR/ABL in GFP⁺ WT and IGF-IR-null leukemia cells as determined by real-time RT-PCR. (B) Representative flow cytometry plots showing that WT and IGF-IR-null BCR/ABL transplanted mice have comparable GFP⁺ cells at 3 months after transplantation. (C) Survival curve of mice receiving BCR/ABL-infected WT or IGF-IR-null hematopoietic progenitors (n = 15). (D) Comparison of the sizes of lungs, livers, and spleens of representative mice transplanted with WT BCR/ABL cells and IGF-IR-null BCR/ABL cells at 3 months after transplantation. (E) Histological analysis of leukemia infiltration in the internal organs of WT and IGF-IR-null BCR/ABL cells transplanted mice at 3 months (hematoxylin/eosin staining).