Figure 4

Lack of IGF-IR decreases self-renewal of CML cells. (A-B) LSK percentages dramatically decreased in leukemia bone marrow from mice transplanted with IGF-IR-null compared to WT BCR/ABL cells as shown in (A) representative flow cytometry plots and (B) as summarized in the bar graph. (C) IGF-IR-null leukemia cells have dramatically decreased CFU forming ability in second replating (n = 3). (D-E) Expression of IGF-IR in WT and IGF-IR-null BCR/ABL leukemia cells as determined by (D) real-time RT-PCR and (E) western blotting.