Figure 1

Trogocytic capabilities of tumor cell lines in vitro . As a representative example, trogocytosis capability results obtained for the U937 cell line are presented. Acceptor cells were U937 cells. Membrane-donor cells were LCL-HLA-G1 cells for allogeneic trogocytosis experiments, and U937-HLA-G1 for autologous trogocytosis experiments. Membrane-donor cells were labeled with the lipophilic dye PKH67 prior to trogocytosis assay. For both allogeneic and autologous trogocytosis experiments, membrane donor and membrane acceptor cells are shown prior to and at the end of the 30-min trogocytosis assay. After the trogocytosis assay, PKH67^hi donor cells were gated out and trogocytic PKH67^low cells and HLA-G1^low cells can be seen. Results shown are representative of three independent experiments.