Trogocytosis capabilities of freshly isolated hematological tumor cells
. As a representative example, trogocytosis capability results obtained for tumor cells from the patient B-CLL 15 are presented. In allogeneic trogocytosis assays, donor cells were PKH67-prelabeled LCL-HLA-G1 cells and acceptor cells were CD19+ B-CLL cells. In autologous trogocytosis assays, donor and acceptor cells were from the same blood sample; donor cells were PKH67-prelabeled and acceptor cells were prelabeled with CD5. For both allogeneic and autologous trogocytosis experiments, membrane donor and membrane acceptor cells are shown prior to and at the end of the 30-min trogocytosis assay. After the trogocytosis assay, trogocytic, PKH67hi donor cells were gated out, and PKH67low cells and HLA-G1low acceptor B-CLL cells can be seen.