ENO1 is highly expressed in NSCLC. (A) The expression of ENO1 mRNA in NSCLC (T) and control tissues (P, N) was analyzed by quantitative RT-PCR. Data show the mean ± SD of three independent experiments (*P < 0.05). (B) The expression of ENO1 protein in NSCLC and non-cancerous lung specimens was analyzed by immunohistochemistry. a, b) Staining of ENO1 in primary NSCLC tissues. c, d) Staining of ENO1 in non-cancerous lung tissues. (C) Immunofluorescence staining of ENO1 in A549 and SPCA-1 cells. (D) Quantitative RT-PCR and Western blot showing the expression of ENO1 mRNA and protein in two NSCLC cell lines and immortalized normal bronchial epithelial cell line (HBE). Bars show the mean ± SD of three independent experiments (*P < 0.05). β-Actin served as protein loading control.