The levels of ENO1 and glycolysis in ENO1-overexpressed and ENO1-suppressed NSCLC cells. (A) Stably upregulated ENO1 by full-length ENO1 increased the expression of ENO1 in A549 cells by qRT-PCR and Western blotting. (B) Stably knocking down ENO1 by shRNA reduced the expression of ENO1 in SPCA-1 cells by qRT-PCR and Western blotting. (C) Transiently knocking down ENO1 by siRNA reduced the expression of ENO1 by qRT-PCR and Western blotting. (D) Western blotting showing the protein expression level of LDHA in ENO1-overexpressed A549 cells and ENO1-suppressed SPCA-1 cells. The histograms showed the relative levels of lactate in NSCLC with ENO1 overexpression and silencing. All of the experiments were repeated at least three times. β-Actin served as a loading control. Bars show the mean ± SD; (*P < 0.05).