Figure 3
ENO1 promotes cell proliferation in vitro and tumorigenicity in vivo . (A) In vitro viability of A549 cell was increased in ENO1-overexpressed cells and was reduced in ENO1-suppressed cells compared to control cells by MTT assay. (B) In vitro viability of SPCA-1 cell was decreased in ENO1-suppressed cells compared to control cells by MTT assay. (C) In vitro proliferative ability of A549 cells was significantly increased in ENO1-overexpressed cells compared to control cells by clone formation assay. (D) In vitro proliferative ability of SPCA-1 cells was significantly decreased in ENO1-suppressed cells compared to control cells by clone formation assay. (E) In vivo tumorigenicity of A549 cells in nude mice was significantly increased in ENO1-overexpressed cells compared to PLV-Ctr cells. In vivo tumorigenicity of SPCA-1 cells in nude mice was significantly decreased in ENO1-suppressed cells compared to PLV-shCtr cells (N = 6 per group). One-way ANOVA was used for MTT assay and plate clone formation. Data are presented as mean ± SD for three independent experiments (*P < 0.05).