ENO1 promotes cell migration and invasion. (A) Stably upregulated ENO1 elevated the migration and invasion of A549 cells in vitro. (B) Transiently knocking down ENO1 reduced the migration and invasion of A549 cells in vitro. (C, D) Stable and transient downregulated ENO1 reduced the migration and invasion of SPCA-1 cells in vitro. (E) External optical fluorescence images of liver were obtained 40 days after spleen injection. Representative photographs of H&E staining of metastatic cancer tissues (M) are shown. Data are presented as mean ± SD for three independent experiments (*P < 0.05).