Figure 7

Ang II treatment reverses the inhibitory effect of stably downregulated ENO1 in SPCA-1 cells. ENO1-suppressed SPCA-1 cells were treated with Ang II (1 μmol/l) for 12 h to activate the phosphorylation of FAK, and cell viability, migration, and invasion were examined by MTT assay, transwell apparatus, and Boyden chamber, respectively. (A) The inhibiting effect of stably downregulated ENO1 in glycolysis was reversed by Ang II treatment. (B) The inhibiting effect of stably downregulated ENO1 in cell proliferation was reversed by Ang II treatment. (C) The inhibiting effect of stably downregulated ENO1 in cell migration and invasion was reversed by Ang II treatment. Data are presented as mean ± SD for three independent experiments (*P < 0.05).