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Figure 2 | Journal of Hematology & Oncology

Figure 2

From: TIM-3/Gal-9 interaction induces IFNγ-dependent IDO1 expression in acute myeloid leukemia blast cells

Figure 2

NK/AML co-culture supernatants induce IFNγ-dependent IDO1 activation. Supernatants obtained from AML alone, stimulated overnight with rh-IFNγ 100 ng/ml and co-cultured with NK cells were used to culture AML blasts that do not spontaneously express IDO1. (A) IDO1 and IRF1 expression was evaluated by western blotting and compared with kynurenine release in the new culture supernatants. GAPDH Ab was used as loading control. Results shown in the figure are representative of three AML samples. (B) ELISA assay for IFNγ quantification was performed in the new three culture conditions. Bars are representative of mean values and standard deviation recorded in three independent experiments performed in duplicate (*P < 0.05; **P < 0.01).

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