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Figure 1 | Journal of Hematology & Oncology

Figure 1

From: The non-peptide thrombopoietin receptor agonist eltrombopag stimulates megakaryopoiesis in bone marrow cells from patients with relapsed multiple myeloma

Figure 1

Human multiple myeloma cells do not express MPL. cDNA (10 ng/sample) prepared from primary CD138+ multiple myeloma cells (four representative patient samples) and from the KMS-11 and OCI-My5 human myeloma cell lines was used as a template to amplify a specific 144 bp fragment of the human MPL gene and a 797 bp fragment of the GAPDH gene by RT-PCR. cDNA prepared from normal CD34+ cells cultured in the presence of 100 ng/ml rhTPO for 4 days or K562 cells were used as positive and negative controls, respectively.

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