P53 plays an important role in oroxylin A-induced suppression of glycolysis. (A, B) Wt-p53 cancer cells (MCF-7 and HCT116) were treated with oroxylin A (100 and 200 μΜ) for 48 h. (A) Glucose uptake was measured using the Amplex Red assay. (B) Production of lactic acid was assayed by Lactic Acid production Detection kit. (C, D) MCF-7 and HCT116 were transfected with siRNA targeting wt-p53 or with a non-targeting control siRNA, then incubated with 200 μM oroxylin A for 48 h. Glucose uptake (C) and lactate production (D) were detected. (E, F) Cells were transfected with a cDNA clone targeting wt-p53, or mut-p53 or with a non-targeting vector. Cells were then incubated with 200 μM oroxylin A for 48 h. Glucose uptake (E) and lactate production (F) were detected. Bars, SD; *p < 0.05 or **p < 0.01 versus non-treated control.