Figure 3

Oroxylin A enhances p53 expression through post-transcriptional regulation. (A) Cells were treated with oroxylin A (100 and 200 μΜ) for 48 h. Western blot assays were performed for MDM2. (B) Effect of oroxylin A on p53 expression after co-treatment with CHX. Cells were treated with vehicle or oroxylin A for 48 h, and 6 h before harvested, 10 μg/ml CHX was added to the medium. p53 protein expression was detected by Western blotting. (C) Effect of oroxylin A on p53 expression after co-treatment with MG132. Cells were treated with oroxylin A for 48 h, and 6 h before harvested, 4 μM MG132 was added to the medium. p53 expression was detected by Western blotting. (D) MDM2 was immunoprecipitated using p53 (Ab6) antibodies. Western blot assays were performed for MDM2, p53. (E) p53 (Ab6) was immunoprecipitated using anti-SIRT3 antibody. Western blot assays were performed for p53 and SIRT3. All the Western blot bands were quantified.