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Figure 4 | Journal of Hematology & Oncology

Figure 4

From: Oroxylin A promotes PTEN-mediated negative regulation of MDM2 transcription via SIRT3-mediated deacetylation to stabilize p53 and inhibit glycolysis in wt-p53 cancer cells

Figure 4

The oroxylin A regulation of p53, MDM2 and p53-related glycolytic pathway components is SIRT3-dependent. (A) Cells were treated with oroxylin A (100 and 200 μΜ) for 48 h. Western blot assays were performed for SIRT3. (B) H1299 cells were transfected with a cDNA clone targeting SIRT3 or with a non-targeting vector, and then incubated with 200 μM oroxylin A for 48 h. Western blot assays were performed for p53, MDM2, and SIRT3. (C) Cells were transfected with siRNA targeting SIRT3 or with a non-targeting control siRNA and incubated with 200 μM oroxylin A for 48 h. Western blot assays were performed for p53, MDM2, and the p53-targeted gene products TIGAR, PGM, and GLUT4. All the Western Blot bands were quantified.

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