Figure 1

Relative ANRIL expression in HCC tissues and HCC cell lines, and ANRIL regulation by SP1. (A) Relative ANRIL expression in HCC tissues (n = 77) compared with corresponding non-tumor tissues (n = 77). ANRIL expression was examined by qPCR and normalized to GAPDH expression. Results were presented as ΔCT in tumor tissues relative to normal tissues. (B) ANRIL expression was classified into two groups. Positive ΔΔCT meant high ANRIL expression. Negative ΔΔCT meant low ANRIL expression. (C) Relative ANRIL expression levels of HCC cell lines (HepG2, Hep3B, MHHC-97H) compared with those in the normal hepatic epithelium cell line (L02). (D) ChIP-qPCR of SP1 occupancy and binding in the ANRIL promoter in HepG2 and Hep3B cells, and IgG as a negative control. (E) The SP1 expression level was determined by qPCR when HepG2 cells were transfected with si-SP1. (F) The ANRIL expression level was determined by qPCR when HepG2 cells were transfected with si-SP1. (G) The SP1 expression level was determined by qPCR when Hep3B cells were transfected with si-SP1. (H) The ANRIL expression level was determined by qPCR when Hep3B cells were transfected with si-SP1. (I) The SP1 expression level was determined by qPCR when HepG2 cells were transfected with EGFP-SP1. (J) The ANRIL expression level was determined by qPCR when HepG2 cells were transfected with EGFP-SP1. (K) The SP1 expression level was determined by qPCR when Hep3B cells were transfected with EGFP-SP1. (L) The ANRIL expression level was determined by qPCR when Hep3B cells were transfected with EGFP-SP1. *P < 0.05, **P < 0.01.