Figure 4

Effects of downregulation of ANRIL on tumor growth in vivo. (A) Tumors from mice 16 days after injection of HepG2 cells stably transfected with sh-ANRIL or empty vector. (B) The tumor volume was calculated every 4 days after injection of HepG2 cells stably transfected with sh-ANRIL or empty vector. Points, mean (n = 5); bars indicate s.d. (C) Tumor weights are represented as means of tumor weights ± s.d. (D) qPCR analysis of ANRIL expression in tumor tissues formed from HepG2/sh-ANRIL and HepG2/empty vector. (E). Tumors developed from sh-ANRIL-transfected HepG2 cells showed lower Ki-67 protein levels than tumors developed by control cells. Left: H&E staining. Right: immunostaining. *P < 0.05, **P < 0.01.