Figure 7
ANRIL negatively regulates expression of KLF2 by rescue assays. (A,B) Colony formation assays were used to determine the cell viability for HepG2 cells transfected with si-NC and si-ANRIL and co-transfected with siANRIL and si-KLF2. Values represent the mean ± s.d. from three independent experiments. (C) MTT assays were used to determine the cell viability for HepG2 cells transfected with si-NC and si-ANRIL and co-transfected with siANRIL and si-KLF2. Values represent the mean ± s.d. from three independent experiments. (D,E) The levels of KLF2 protein levels were determined by Western blot when HepG2 cells were transfected with si-NC and si-ANRIL and co-transfected with siANRIL and si-KLF2.*P < 0.05, **P < 0.01.