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Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: Angptl4 is upregulated under inflammatory conditions in the bone marrow of mice, expands myeloid progenitors, and accelerates reconstitution of platelets after myelosuppressive therapy

Fig. 5

Angptl4 accelerates the reconstitution of megakaryopoiesis after lethal irradiation and transplantation. a Reconstitution of PLTs, erythrocytes (RBCs), and leucocytes (WBCs) after lethal irradiation (2 × 6.5 Gy) and transplantation of 1 × 105 BM cells. After transplantation, mice were daily injected with PBS (shown in squares) or murine Angptl4 (shown in circles; 250 μg/kg body weight) for five consecutive days. Peripheral blood of transplanted mice was collected every 3 days for a time course of 30 days, and PLT, RBC, and WBC counts were determined using an animal blood counter. Each time point represents the mean of two different experiments with a total of ten mice/group. b PLT, RBC, and WBC counts after lethal irradiation (2 × 6.5 Gy) and transplantation of 1 × 105 BM cells from Angptl4 (circles) or PBS (squares) pre-treated donor mice. Donor mice were daily injected with PBS or Angptl4, as described in a and sacrificed 48 h after the last injection. After transplantation, peripheral blood was collected and analyzed as described in a. Each time point represents the mean of two different experiments with a total of ten mice/group. c Frequency and total cell numbers of CD61+, CD61+CD41high, and CD61+CD41low cells in the spleen at day 30 after transplantation. Recipient mice were treated with Angptl4 after transplantation, as described in a. Mean ± SEM of three different experiments with three PBS-injected (white bars) and three Angptl4-injected (gray bars) mice/group are shown. d Frequency and total cell numbers of CD61+, CD61+CD41high, and CD61+CD41low cells in the BM at day 30 after transplantation. Recipient mice were treated with Angptl4 after transplantation, as described in a. Mean ± SEM of three different experiments with three PBS-injected (white bars) and three Angptl4-injected (gray bars) mice/group are shown. (*p < 0.05, **p < 0.01)

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