Fig. 5From: Angptl4 is upregulated under inflammatory conditions in the bone marrow of mice, expands myeloid progenitors, and accelerates reconstitution of platelets after myelosuppressive therapyAngptl4 accelerates the reconstitution of megakaryopoiesis after lethal irradiation and transplantation. a Reconstitution of PLTs, erythrocytes (RBCs), and leucocytes (WBCs) after lethal irradiation (2 × 6.5 Gy) and transplantation of 1 × 105 BM cells. After transplantation, mice were daily injected with PBS (shown in squares) or murine Angptl4 (shown in circles; 250 μg/kg body weight) for five consecutive days. Peripheral blood of transplanted mice was collected every 3 days for a time course of 30 days, and PLT, RBC, and WBC counts were determined using an animal blood counter. Each time point represents the mean of two different experiments with a total of ten mice/group. b PLT, RBC, and WBC counts after lethal irradiation (2 × 6.5 Gy) and transplantation of 1 × 105 BM cells from Angptl4 (circles) or PBS (squares) pre-treated donor mice. Donor mice were daily injected with PBS or Angptl4, as described in a and sacrificed 48 h after the last injection. After transplantation, peripheral blood was collected and analyzed as described in a. Each time point represents the mean of two different experiments with a total of ten mice/group. c Frequency and total cell numbers of CD61+, CD61+CD41high, and CD61+CD41low cells in the spleen at day 30 after transplantation. Recipient mice were treated with Angptl4 after transplantation, as described in a. Mean ± SEM of three different experiments with three PBS-injected (white bars) and three Angptl4-injected (gray bars) mice/group are shown. d Frequency and total cell numbers of CD61+, CD61+CD41high, and CD61+CD41low cells in the BM at day 30 after transplantation. Recipient mice were treated with Angptl4 after transplantation, as described in a. Mean ± SEM of three different experiments with three PBS-injected (white bars) and three Angptl4-injected (gray bars) mice/group are shown. (*p < 0.05, **p < 0.01)Back to article page