Fig. 2
From: Evaluation of targeting c-Src by the RGT-containing peptide as a novel antithrombotic strategy
The RGT sequence of the β3 tail was not necessary for the direct phosphorylation of the β3 Y747 and Y759 by active c-Src. a The amino acid sequences for the integrin β3-ΔRGT cytoplasmic peptides, or with Y759F, or Y747F mutations. b MALDI-TOF-MS results of the differently mutated β3 cytoplasmic peptides incubated with or without active c-Src. Black and blank arrows denote the new peak with one and two phosphorylated tyrosine(s), respectively. c [γ-32P]ATP incorporation of differently mutated β3 cytoplasmic peptides by active c-Src. Results are presented as mean and SD of three independent experiments. d Western blot analysis of the tyrosine phosphorylation of Tac-β3-ΔRGT. The phosphorylation level of Y747 residue in CHO cells expressing Tac-β3-ΔRGT or Tac-β3 together with activated c-Src was similar, but stronger than in those expressing only Tac-β3-ΔRGT or Tac-β3