Fig. 5
From: Evaluation of targeting c-Src by the RGT-containing peptide as a novel antithrombotic strategy
Myr-AC ~ CRGT inhibited the phosphorylation of the integrin β3 cytoplasmic tyrosines in thrombin-stimulated platelets. a Effect of myr-AC ~ CRGT on c-Src activity in platelets in response to thrombin stimulation. After an incubation with myr-AC~CRGT or myr-AC~CGRT, platelets were stimulated by thrombin then lysed and blotted with anti-Src-pY416, anti-Src-pY527, and anti-Src antibodies. Integrin β3 served as a loading control. Results are shown as the mean and SD of three independent experiments. b Effect of myr-AC ~ CRGT on thrombin-induced phosphorylation of the Y747 and Y759 of the β3 cytoplasmic tail. After an incubation with 250 μM of myr-AC ~ CRGT or myr-AC ~ CGRT, platelets were stimulated by thrombin (0.05 U/mL) with stirring, then were lysed in SDS-PAGE sample buffer and analyzed by Western blot using anti-β3, anti-β3-pY747, anti-β3-pY759, or anti-β-tubulin antibodies. Untreated platelets were used as a negative control, thrombin-stimulated platelets as a positive control, and β-Tubulin as a loading control