Fig. 6
From: Evaluation of targeting c-Src by the RGT-containing peptide as a novel antithrombotic strategy
Effect of myr-AC ~ CRGT on thrombus formation of human platelets at different shear rates. Calcein AM-labeled whole blood was incubated with DMSO, myr-AC ~ CRGT (250 μM), or 7E3 prior to perfusion, and then perfused at different wall shear rates (5000, 1500, 500, or 125 s−1) for 4 min. The thrombus formation was observed and imaged under an inverted fluorescent microscope. a The representative images showed platelet thrombi (scale bar is 100 μm). b The quantitative data were acquired as platelet-integrated total fluorescence intensity. c The percentage of surface coverage. d The mean fluorescence intensity calibrated by surface coverage. Results are the mean and SD from three independent experiments, and 40 visual fields were randomly chosen for each experiment. **P > 0.05; *P < 0.01