Fig. 3
From: Targeting Wnt pathway in mantle cell lymphoma-initiating cells
Enrichment of Wnt signaling pathway genes in MCL-ICs. a Expression of Wnt3 in unfractionated MCLs (n = 20) and MCL-ICs isolated from unfractionated MCLs expressing high (n = 3) and low (n = 3) Wnt3. b Expression of mRNAs encoding Wnt ligands and FZD receptors in freshly isolated MCL-ICs and MCL-non-ICs relative to B-cells from healthy donors. Horizontal lines represent median for each group. Differences between MCL-ICs and MCL-non-ICs were significant (P < 0.05) for Wnt3, Wnt7b, FZD1, FZD5, FZD9, and FZD6. c Immunostaining detection of the expression and localization of β-catenin in freshly isolated MCL-ICs and MCL-non-ICs. Color image is included in Additional file 1: Figure S1. d Relative expression levels of Wnt target genes encoding ID2 and β-catenin–interacting transcriptional factor TCF4 in MCL-ICs (n = 4) and MCL-non-ICs (n = 4). Differences between MCL-ICs and MCL-non-ICs were significant (P < 0.05) for both genes