Fig. 6

Knockdown of SAE2 sensitized chemotherapy in H446 (a). shCtrl-H446 and shSAE2-H446 tumor cells were treated with etoposide (15 μM) or cisplatin(1.5 μM) for 24 and 48 h, respectively. Cell apoptosis was detected by Annexin V and propidium iodide staining (b). The FCM results are presented as the percentage of apoptotic cells. Drug concentration–dependent cell survival curves for etoposide (c) for 48 h and cisplatin (d) for 72 h in shSAE2-H446cells or shCtrl-H446 cells. Each data point represents means ± SD of three independent experiments (*P < 0.05, **P < 0.01)