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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: Evaluation of hGM-CSF/hTNFα surface-modified prostate cancer therapeutic vaccine in the huPBL-SCID chimeric mouse model

Fig. 2

Immunohistochemical staining analysis of lymphocytes in tumor tissue and assessment of PC-3-specific cytotoxicity and IFNγ in spleen. Immunohistochemical staining analyzed CD4+ or CD8+ lymphocytes in the tumor tissues from huPBL-SCID mice 8 weeks after vaccination. The images of immunohistochemical staining were shown with ×200 magnification. Tumor tissues from different groups were stained with anti-hCD4 or anti-hCD8 antibody (a), and the quantitative analysis of the images was performed with integrated optic density (b). For PC-3-specific cytotoxicity assay, spleen cells were isolated on day 21 after tumor injection from each experimental group. Effector cells were stimulated by recombinant human IL-2 and mitomycin-treated PC-3 cancer cells. The supernatants were collected for the non-radioactive cytotoxicity assay (c). For quantification of IFNγ by ELISA, splenocytes were isolated from experimental mice 7 days after the last tumor vaccination and incubated with hIL-2 and mitomycin-treated PC-3 cancer cells for 48 h. The supernatants were collected for the measurement of IFNγ by ELISA (d). Error bars represented the SEM in both (c) and (d)

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