Fig. 2

The combination of vincristine and SAHA had synergic effects on cell cycle kinetic changes. a MOLT-4 cells were treated with various concentrations of vincristine alone or in combination with SAHA (500 nM) for 24 and 48 h. b The quantitative data are shown in the time course. Cell cycle distribution was performed by cell cytometry after propidium iodide staining. c The combination effect of SAHA (500 nM) and vincristine (0.3, 1, and 3 nM) on G₂/M arrest (left figure) and apoptosis (right figure) were used by the combination index (CI). And the CI was analyzed with the program CompuSyn (Ting-Chao Chou and Nick Martin). A CI value <1 represents that two drugs have synergic effects. d CCRF-CEM cells were treated with the indicated concentrations of vincristine alone or in combination with SAHA (1000 nM) for 48 h. The effect of sub-G₁ phase was analyzed by flow cytometry. Data are expressed as mean values ± SD of at least three separate determinations. Fa fraction affected, S SAHA, V vincristine