Fig. 2

grp94 is highly expressed in plasma cells. a Q-RT-PCR analysis of grp94 mRNA in CD138⁺ plasma cells and CD138⁻ non-plasma cells. 18S ribosomal RNA was used as an internal control. The quantity of transcripts for respective genes was expressed as a relative fluorescence unit (RFU), calculated based on the number of cycles to reach the threshold of detection (Ct) using the formula of 2^ΔCt (Ct _interest − Ct _{18S rRNA}). b Western blot for grp94 of CD138⁺ plasma cells and CD138⁻ non-plasma cells. β-actin was blotted to indicate equal loading of cell lysates