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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: TMEM140 is associated with the prognosis of glioma by promoting cell viability and invasion

Fig. 2

Suppressing of TMEM140 expression by RNAi. a TMEM140 expression level in five glioma cell lines was analyzed by RT-PCR (left) and immunoblot (middle and right). b, c The effect of TMEM140 knockdown through siRNA silencing. The cells were transfected with normal control or TMEM140-RNAi for 48 h and then subjected to RT-PCR (left) and immunoblot analysis (middle and right) of the TMEM140 expression level. The representative images for immunoblot are shown in the middle panel, and data from three independent experiments were expressed as the mean ± S.D. (right panel). Wild type: wild-type cells; normal control: scrambled siRNA transfected cells; RNAi-1, RNAi-2, and RNAi-3: TMEM140-RNAi-1, -2, and -3 transfected cells (*P < 0.05, **P < 0.01, ***P < 0.001 compared with normal control)

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