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Fig. 4 | Journal of Hematology & Oncology

Fig. 4

From: Genetically modified "obligate" anaerobic Salmonella typhimurium as a therapeutic strategy for neuroblastoma

Fig. 4

Sal-YB1 could enhance the anti-neuroblastoma effect of macrophages via TNFα. TNFα ELISA assay quantified the Sal-YB1 targeting effect on both a mouse tumor tissues and b human macrophages. Relatively, the expression of TNFα in nude mice and 2-day hypoxia-cultured macrophages were significantly higher than in NOD-SCID mice and nomoxia-cultured macrophages, respectively (P < 0.05). The error bars showed means ± SEM for three mice in (a) and three healthy human donators in (b). *P < 0.05 by an unpaired two-tailed t test. c TNFα and pan-caspase inhibitors could reverse the anti-neuroblastoma effect by macrophages. The viability of neuroblastoma was evaluated by XTT test. Pan-caspase inhibitor blocked the apoptosis of neuroblastoma both in Sal-YB1 (P < 0.05) and 50 % supernatant of the Sal-YB1 activated macrophages (P < 0.001)-treated group. Anti-TNFα significantly rescued neuroblastoma co-cultured with the supernatant from YB1 pre-treated macrophages (P < 0.01). The error bars showed means ± SEM in triplicate experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 by an unpaired two-tailed t test

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